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- AutoSpill compensation: AutoSpill is a new algorithm for calculating spillover and producing a compensation matrix developed and incorporated into FlowJo v10.7 in collaboration with researchers from the Vlaams Instituut voor Biotechnologie in Belgium and the the Babraham Institute in Cambridge, UK.
- Our exclusive AutoSpread algorithm provides a Spillover Spreading Matrix (SSM) by calculating spillover spreading coefficients with linear models, removing the need for well-defined positive and negative populations in single-color controls.
'Together, AutoSpill and AutoSpread remove limiting constraints of traditional compensation methods, easing the preparation of compensation controls in high-parameter flow cytometry, making errors less likely, and facilitating the practical implementation of ultra high-parameter flow cytometry.' —Roca, et al
Read the full article
AutoSpill: a method for calculating spillover coefficients in high-parameter flow cytometry.
Authors: Carlos P. Roca, Oliver T. Burton, Teresa Prezzemolo, Carly E. Whyte, Richard Halpert, Łukasz Kreft, James Collier, Alexander Botzki, Josef Spidlen, Stéphanie Humblet-Baron, Adrian Liston. - Proliferation and Cell Cycle models have been updated to better fit data with missing peaks.
- The t-SNE derived parameters now remain connected to workspace files after saving. Note: In case derivative files are ever dissociated from a workspace, drag and drop the CSV back onto the data file or right click and choose “Import Parameters from CSV/CLR”.
- Figures added to the Layout Editor will no longer lose parameter annotations without user deselecting those features manually or setting them hidden in the preferences.
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